Application area
ELISA-IFA
Product line
Automation
APE IF
ELITE
is the latest version of combined ELISA and IFA automated processors of DAS. The instrument is able to manage 10 methods on line with 4 elisa microplates at different temperature; a thre needle system allows fast processing on 16 IFA slides (optional 20 slides). It is equipped with a sliding sample barcode reader; a microplate shaker is also available.
All parameter for dilution, dispense, incubation, washing, reading and data processing, are settable.
TECHNICAL FEATURES
| ELISA technical features | |
| Liquid handling | 2 probes for dilution and dispense of samples and reagents |
| Sample dispense time | 6s (average) with 2ml of washing solution |
| Sample dilution time | 6s (average) with 2ml of washing solution |
| Plate washing | two 8 nozzle manifolds, one to dispense and the other one to aspirate; residual volume <3µl |
| Plate washing inaccuracy | CV <5% at 300µl |
| Removable tray | modular racks to hold calibrators, controls and reagents for 10 methods on line |
| Microtiter plates | 4 standard plates (12 x 8 wells format) independently incubated at room temperature or from 37° to 45° C |
| Wash buffers | 4 wash buffer for microplates in external tanks |
| Reading system | 8 independent photometric channel with mono and bichromatic reading capability |
| Interferential filters | 2 fprecision filters (450 and 630 nm); 2 filters more on request (4 filter max, 400-700 nm) |
| Reading inaccuracy | Reading from 0.000 to 3.000 OD; inaccuracy ±2% |
| Operative Functions | Management of 10 methods in the same run. Definition of incubation times and volume for sample and reagents dispense. Differen incubation times for each method. Easy and fast settings for new protocols. Work list set up, patient reports and result fling. Calibration curve storage for each carried out test |
| IFA technical features | |
| Liquid handling | 3 probes: two to dispense and dilute sample and reagents , the third one to aspirate from wells |
| Average thoughput | 320 wells in a run with incubation times of 30 + 30 minutes |
| Sample dispense time | 6s (average) with 2ml of washing solution |
| Sample dilution tiem | 15s with 2 ml of washing solution |
| Slide washing | Wells are washed one by one to avoid carry-over |
| Washing mode | multi-well, continuous flow or drop by drop for specific washing effectiveness |
| Supllied trays | Tray with 16 position for IFA slides (optional 20 slides); reagent tray for 16 controls, 12 conjugates (11 in case of Blue Evans dye) |
| Wash buffers | 2 wash buffers for slides in external tanks |
| Operative functions | Management of 10 methods in the same run. Definition of incubation times and volume for sample and reagents dispense. Differen incubation times for each method. Easy and fast settings for new protocols. Slide mask, number of wells and thickness for each slide; shape, size and coordinates for each slide well. maschera vetrini, numero di pozzetti e spessore di ogni vetrino; forma, dimensione e coordinate per ogni pozzetto del vetrino |
| Caratteristiche tecniche COMUNI | |
| Built-in barcode reader | for sample; barcode reader for slides (optional) |
| Sample tray | 169 sample tubes Ø13mm or 130 sample tubes Ø16mm; combination of them can be used in the same run |
| Rack diluizioni | 264 dilution cups |
| Dilution tray | 4 sample diluents on board |
| Dispensation | CV <0.1% inaccuracy 1% at 100µl |
| Aspiration | CV <0.3% inaccuracy1% at100µl; minimum volume 5µl, resolution 1µl |
| Syringes | 2 with 1000µl volume each, 1µl resolution |
| External liquid tanks | 8 external tanks with level sensors |
| Operative functions | Management of 10 methods in the same run. Definition of incubation times and volume for sample and reagents dispense. Differen incubation times for each method. Easy and fast settings for new protocols. |
| Connections | LIS interface capability, work list import from Host Computer; a single PC can run more instrument at the same time. |
| PC | Minimum requirements: 250MB free spache Hard Disk; CD-Rom reader; 2GB RAM; Intel Pentium Dual Core or equivalent; VGA 1024 x 768 x 16 million colours; 1 free USB 2.0 port; printer Suggestedi requirements: 4GB RAM; Intel Core 2 Duo E6500 series or equivalent |
| Operative systems | Windows 7 or Windows 10 |
| Power requirements | 230 o 115 VAC, 50-60 Hz, 650 VA |
| Measurements | 113 x 77 x 75 h |
| Weight | 120 Kg |
