Technologies and Innovation
with DAS Instruments since 1977
ELISA - IFA - BLOT AP22 IF BLOT ELITE
Product Line
Automatic Processor ELISA – IFA – BLOT
AP22 IF BLOT ELITE is a tool for the automation of ELISA, IFA and BLOT methods. Capable of running 2 ELISA microplates with 8 in-line tests, 16 IFA slides or 24 BLOT strips; in addition to the ELISA and IFA processes (AP22, AP22 IF) it is able to perform, thanks to the Blot module and the chamber, complete automation for Dot/Western Blot starting from the dilution and dispensing of samples, reagent dispensing, incubation and strip washing up to image processing and evaluation for each strip, the software also provides for the creation of reports and their archiving.
TECHNICAL SPECIFICATIONS
| ELISA Technical Features | |
| ELISA Dilution Cups | 110 positions |
| Removable Trays | modular trays for calibrators, controls, and reagents for 8 methods in line |
| Microplates | 2 standard plates (12 x 8 wells) incubated at room temperature or from 36° to 45°C |
| Shaker | Microplate shaker (optional) |
| Liquid Handling | 1 needle for dilution and dispensing, the second for washing the wells of the slides |
| Sample Dispensing Time | 11s with 2ml of wash |
| Microplate Washing | 2 manifolds with 8 needles, one for dispensing and the other for aspiration; residual volume <3µl |
| Wash Buffer | 2 wash buffers for ELISA microplates in external tanks with level sensors |
| Washing Accuracy | CV <5% with 300 µl |
| Reading System | 8 independent photometric channels for mono or bicromatic reading |
| Interference Filters | 2 precision filters (450 and 630 nm); another 2 on request (4 filters max; 400-700 nm) |
| Reading Accuracy | Reading from 0.000 to 3.000 OD; inaccuracy ± 1% from 0.000 to 1.500 OD ± 2% from 1.500 to 3.000 OD |
| IFA Technical Features | |
| Liquid Handling | 2 needles: one for dilution and dispensing, the other for slide washing |
| Sample Dispensing Time | 11s with 2 ml of wash |
| Slide Process Time | 30 minutes to dispense or wash 16 slides with 12 wells |
| Provided Trays | Capacity for 10 tests in line; tray with 220 positions for dilutions; tray with 16 positions for IFA slides; tray for reagents for 18 controls, 9 conjugates, 1 wash solution for the needle, and two diluents for the samples |
| Liquid Sensor | Able to capture 150µl |
| Wash Buffer | 2 wash buffers for IFA slides in external tanks with level sensors |
| Washing Modes | each well is washed individually to avoid contamination, continuous flow or multi-well for specific washing effectiveness |
| BLOT Technical Features | |
| Liquid Handling | 2 needles: one for dilution and dispensing, the other for strip washing |
| Dispensing | up to 3000µl with a resolution of 1 µl |
| Reagent Tray | 10 tests in line: 10 x 110 ml reagent bottles and 10 positions for controls |
| Strip Tray | 3 trays (8 strips per tray) to perform 24 strips on the oscillating plane |
| Wash Buffer | 2 wash buffers for strips in external tanks with level sensors |
| Reading Capacity | high-resolution color camera for image acquisition |
| Common Technical Features | |
| Barcode Reader | onboard barcode reader for samples |
| Sample Tray | 91 sample tubes Ø13mm or 70 sample tubes Ø16mm that can be used in combination |
| Dilution Cups | Blocks of 22 cups to facilitate loading |
| Syringe | 1000µl volume with a resolution of 1µl |
| Dispensing | from 8µl to 1000µl with a step of 1µl; inaccuracy ±1% at 100µl |
| Liquid Container | External Wash and Waste tanks equipped with level sensors |
| Connections | Possibility of LIS interfacing and loading worklist from Host computer. Possibility of connecting multiple instruments to a single PC. |
| Operating Systems | Windows 7 or Windows 10 |
| PC | Minimum requirements: 250 MB free space on Hard Disk; CD-Rom reader; 2GB RAM; Intel Pentium Dual Core or equivalent, VGA 1024 x 768 16 million colors; 2 free USB 2.0 ports; printer.
Suggested requirements: 4GB RAM; Intel Core 2 Duo E6500 series or equivalent |
| Power Supply | 230 / 115 VAC, 50-60 Hz, 350 W |
| Dimensions | cm 62 x 83 x 72h |
| Weight | 85 Kg |